Increased cloning efficiency by cycle restriction−ligation (CRL)

Increased cloning efficiency by temperature-cycle ligation.

Molecular cloning is a crucial, and often speed-limiting, step in many standard procedures of molecular biology. Ligation of cohesive DNA ends is normally carried out at 12–16 C to ensure a good balance between enzyme activity and stability of annealed DNA overhangs. Low temperatures generally reduce ligase activity, whereas too high temperatures may reduce cloning efficiencies by melting annea...

Mutation and cloning efficiency.

Increased efficiency of cloning large DNA fragments using a lower copy number plasmid.

ow. 1992. Baculovirus Expression Vectors: A Laboratory Manual. Freeman, New York. 14.Paciotti, G.F. and L. Tamarkin. 1988. Interleukin-2 differentially affects the proliferation of a hormone-dependent and a hormone-independent human breast cancer cell line in vitro and in vivo. Anticancer Res. 8:1233-1239. 15.Pham, M.Q., S. Naggie, M. Wier, H.J. Cha, and W.E. Bentley. 1999. Human interleukin2 p...

Improving the Efficiency of Depth-First Search by Cycle Elimination

An experimental evaluation of a technique for improving the efficiency of depth-first search on graphs is presented. The technique, referred to as cycle checking, prevents the generation of duplicate nodes in the depth-first search of a graph by comparing each newly generated node to the nodes already on the search path. Although cycle checking can be applied to any depth-first type search, thi...

Increased efficiency of arbitrarily primed PCR by prolonged ramp times.

for functional analysis. Mutagenesis to the small promoter-only containing plasmid is preferred to that of the reporter gene construct, because amplification of a smaller plasmid is faster and more efficient and removes the slight possibility of introducing random mutations to the reporter gene. The transferred mutant region can be sequenced to confirm that no random mutations were introduced d...